MMOBILIZATION OF SACCHAROMYCES ON NATA DE COCO: EFFECTS OF AGITATION RATE AND NATA DE COCO GEOMETRY: Difference between revisions
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Many industrial process utilize biological reactors (bioreactors) for the manufacture of various products. Increasing biocatalyst (cells, enzymes, etc.) concentrations have been shown to be an effective way of improving the performance of bioreactors. | Many industrial process utilize biological reactors (bioreactors) for the manufacture of various products. Increasing biocatalyst (cells, enzymes, etc.) concentrations have been shown to be an effective way of improving the performance of bioreactors. To achieve this, immobilization of biocatalysts is done by various mechanisms and using various materials. Nata de coco (NDC), a variant of microbial cellulose derived from coconut milk, is one such material that may be used for immobilization. Sacchararomyces cerevisiae was immobilized onto NDC and the effects of agitation rate and NDC geometry during immobilization were determined. A 2-way analysis of variance shows that both factors had a significant effect on the immobilization of yeast. The smaller sized NDC geometries agitated at 50 rpm produced the best results with cell numbers as high as 1.9 x 108 cells per gram of material. Higher agitation rates (>100 rpm) inhibited immobilization due to the reduced contact time between cells and immobilizing material. | ||
[[Category: Theses]][[Category: Chemical Engineering Thesis]] | [[Category: Theses]][[Category: Chemical Engineering Thesis]] | ||
Revision as of 12:14, 7 September 2011
Miguel Carlo Hipolito
(MS Graduated: 2nd Sem 2010-2011)
Abstract
Many industrial process utilize biological reactors (bioreactors) for the manufacture of various products. Increasing biocatalyst (cells, enzymes, etc.) concentrations have been shown to be an effective way of improving the performance of bioreactors. To achieve this, immobilization of biocatalysts is done by various mechanisms and using various materials. Nata de coco (NDC), a variant of microbial cellulose derived from coconut milk, is one such material that may be used for immobilization. Sacchararomyces cerevisiae was immobilized onto NDC and the effects of agitation rate and NDC geometry during immobilization were determined. A 2-way analysis of variance shows that both factors had a significant effect on the immobilization of yeast. The smaller sized NDC geometries agitated at 50 rpm produced the best results with cell numbers as high as 1.9 x 108 cells per gram of material. Higher agitation rates (>100 rpm) inhibited immobilization due to the reduced contact time between cells and immobilizing material.